FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36099336
Sequences flagged as poor quality0
Sequence length150
%GC33

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7527152.085121454865541Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6997401.9383736033261112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1980210.5485447156147137Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1827770.5063167920872561No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1805440.500131082743461Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT929170.2573925459459975No Hit
ACAGCAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT885070.24517625476546162No Hit
ACAGCAGATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT795870.2204666589989356No Hit
ACAGCAGACGTCTGACCCATGTACTCTGCGTTGATACCACTGCTTCCCAT780280.21614802000790262Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG749830.2077129618118184Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT734170.20337493188240358No Hit
ACAGCAGATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675550.18713640605467094No Hit
ACAGCAGATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598750.16586177651577858No Hit
ACAGCAGATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543740.15062326908173604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG542660.15032409460384533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489300.13554265928880244No Hit
ACAGCAGATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467230.12942897343042542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT450270.12473082607392004Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439330.1217002994182497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC428150.11860328954527032Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394170.10919037402793226No Hit
ACAGCAGATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387680.10739255702653368No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC383650.10627619300255273Clontech Universal Primer Mix Long (96% over 26bp)
ACAGCAGATTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377010.10443682399033599No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC375310.10396590120106365Clontech Universal Primer Mix Long (96% over 26bp)
ACAGCAGATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363220.10061680912912083No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA361460.10012926553552122Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)