FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24718426
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT8727243.530661701517726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG3739661.5129037746982756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1415460.5726335487542775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1087980.44014938491633726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT641580.2595553616561184Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT630730.25516592359076584Clontech SMART CDS Primer II A (96% over 26bp)
CTGAGCCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536560.21706883763553553No Hit
CTGACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT469290.18985432163034976Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA330680.13377874464984138Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295600.11958690249937437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA278600.11270944193614917Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261030.10560138416580407No Hit
CTGAGCCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256820.10389820128514654No Hit
CTGAGCCATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252060.10197251232744352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)