FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002373516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29750466
Sequences flagged as poor quality0
Sequence length150
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT7555442.539603917464688Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2907440.9772754483912958Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1671810.5619441389590334No Hit
AACCGAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1192080.40069288326441677No Hit
AACCGAGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1176800.39555682926109464No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1040650.34979284021971285Illumina Single End PCR Primer 1 (100% over 50bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC938920.31559841785335396Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860230.2891484119946222No Hit
AACCGAGATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT806980.2712495326963954No Hit
AACCGAGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT773670.26005306942082856No Hit
AACCGAGATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT732460.2462011855545389No Hit
AACCGAGATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT662920.22282676177240385No Hit
AACCGAGATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650640.21869909533517895No Hit
AACCGAGATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT645860.21709239781319728No Hit
AACCGAGATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT644750.2167192944137413No Hit
AACCGAGATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624820.21002023968296832No Hit
AACCGAGAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612490.20587576678630848No Hit
AACCGAGATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT566070.1902726498468965No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT563960.18956341725874143No Hit
AACCGAGATATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT535800.1800980193049749No Hit
AACCGAGAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505260.16983263388210457No Hit
AACCGAGATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502020.1687435753107195No Hit
AACCGAGATTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501190.16846458808409925No Hit
AACCGAGATTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491750.1652915285427798No Hit
AACCGAGATTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489200.1644343991115971No Hit
AACCGAGATTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468720.15755047332703967No Hit
AACCGAGATATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466160.15668998260396996No Hit
AACCGAGAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453940.15258248391806703No Hit
AACCGAGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440410.1480346559949683No Hit
AACCGAGATATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436590.14675064249413775No Hit
AACCGAGATTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433630.14575570009558841No Hit
AACCGAGATATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432860.14549688062029012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA430700.1447708415727001Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421960.14183307246347No Hit
AACCGAGATTTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417830.1404448589141427No Hit
AACCGAGATTTTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417630.1403776330764029No Hit
AACCGAGATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416710.14006839422279974No Hit
AACCGAGAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416420.13997091675807702No Hit
AACCGAGATTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409090.13750708980491264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG400500.13461974007398742Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400010.13445503677152484No Hit
AACCGAGATTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399780.13437772705812406No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA399020.13412226887471274Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTTTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391720.13166852579720936No Hit
AACCGAGAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387870.13037442842071786No Hit
AACCGAGAATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384290.1291710859251751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT381780.12832740166154036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377690.12695263327976106No Hit
AACCGAGATTTATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371760.12495938719077543No Hit
AACCGAGATTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371050.12472073546679907No Hit
AACCGAGATTTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362730.12192414061682261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG353470.11881158432946899Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349560.11749731920165554No Hit
AACCGAGATTATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349430.11745362240712465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA345380.11609229919289331No Hit
AACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG343310.11539651177228619Clontech Universal Primer Mix Long (96% over 26bp)
AACCGAGATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342340.11507046645924807No Hit
AACCGAGAAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333030.1119411037124595No Hit
AACCGAGAAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315850.10616640425060905No Hit
AACCGAGATGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313590.10540675228414909No Hit
AACCGAGAAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298780.1004286789995155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)