FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31161285
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT10798323.465299970781051Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2999540.9625854646238113Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1563790.501837456317992Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG879000.28208079352311693Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT801230.2571235428834209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG692050.22208647685742097Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA674430.2164320245458427Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT648460.20809796515130877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC600440.19268781759160444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC591610.18985417321525733Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT585460.1878805703936792Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA511210.16405292657218723Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT482810.154939053379859Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAACGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479300.15381265567193395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT433070.13897693885216866Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG401350.1287976410472161Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAACGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354140.11364743142010991No Hit
GCTAACGATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342490.10990881794508796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)