FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37120716
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT13120903.5346570362489773Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2234920.602068128211751Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1061040.28583500382912874Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT725940.19556196060442368No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG704770.1898589456087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA624890.16833996413215738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT605060.16299793355279033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC540670.14565182417278805Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG508500.13698550426667416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG504790.1359860623378062Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA444010.1196124557511229Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT441460.11892550779462337Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT422800.11389866510117962Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTAGGAATAATGGAATAG419480.11300428580095276Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)