FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39660849
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT13452533.391891585578514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1695480.4274946307881609Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1267060.319473746010833Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT951810.23998729830518756No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG703830.17746216174041055Illumina Single End PCR Primer 1 (100% over 50bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG685830.17292368098322858Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG683740.17239671293975578Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT616100.1553421108055453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT607960.15328970895201965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC580160.14628027756037196Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG529440.1334918473379125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT508300.1281616538264221No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA498990.12581425072367966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT495190.12485612700827459Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG417350.10522971911166097Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416880.10511121433633457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT401330.10119047123776902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400060.10087025620656785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA396690.10002055175369544Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)