FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31985620
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT10787703.3726718444100814Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1044210.32646232900909844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC945070.29546715055077877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG867140.27110307694520225Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG791730.24752685738153582Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT517370.16175081177103962No Hit
ACAGCAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490550.153365793753568No Hit
ACAGCAGATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450480.14083828920621205No Hit
ACAGCAGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402120.12571899497336617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA394310.12327727272443054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380540.118972213138279No Hit
ACAGCAGATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353890.11064034400458705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA325300.10170195231482147Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)