FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41272859
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT8547182.0708960336379896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5919131.434145863265736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1087170.26341039277167594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1011980.2451926095064071Clontech Universal Primer Mix Long (96% over 26bp)
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT791640.191806436282982Clontech Universal Primer Mix Long (96% over 26bp)
CAACCACAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT693080.16792633628797074No Hit
CAACCACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT659140.1597030145161497No Hit
CAACCACATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570390.13819977918176204No Hit
CAACCACATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559880.13565331153821936No Hit
CAACCACATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT541520.13120486758622657No Hit
CAACCACATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491940.11919213059604133No Hit
CAACCACATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464100.11244677767537256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA417250.10109549231857187Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA415070.10056730017176663Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)