FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34406480
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8517972.4756877192900872Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT3161730.9189344565326065Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1550370.4506040722561564Clontech Universal Primer Mix Long (96% over 26bp)
CAATGGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT622470.18091650177524699No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA493440.14341484511057218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATGGAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454730.13216405746824436No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC445860.12958605471992485Clontech Universal Primer Mix Long (96% over 26bp)
CAATGGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443330.12885072811865672No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA420210.12213106368335266Clontech Universal Primer Mix Long (96% over 26bp)
CAATGGAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414840.12057031117394165No Hit
CAATGGAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405840.11795452484531983No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA378030.10987174508987842Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT376320.10937474568744027Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG370980.10782271246579132Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATGGAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354460.10302129133814328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)