FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35206251
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG12663463.59693510109895Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9157892.6012113587442185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6936171.9701529708460013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG4441771.2616424282153758Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2847340.8087597853006275Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1431580.40662665274981996Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCACCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT1405420.39919615411479054Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1212000.3442570468522763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT753740.2140926621241211Clontech Universal Primer Mix Long (96% over 26bp)
AGCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT653120.18551251026415735Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA644300.18300727333904424Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC623010.17696005178171342Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC570930.16216722422390276Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA563880.16016473892661845Clontech Universal Primer Mix Long (96% over 26bp)
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA547060.15538717825990617Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT498120.14148623777067315Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT476620.13537936771512535Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC461310.13103070815463994Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC443500.12597194742490475Clontech Universal Primer Mix Long (96% over 26bp)
AGCACCCCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGT442590.12571347059929783Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA416900.11841647098408746Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC408530.11603905226943931Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC396450.11260784341962453Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG396320.11257091815882354Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT394470.11204544329357874Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC392380.11145179871608596Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA382530.10865400010924195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT368960.10479957096255435Clontech SMART CDS Primer II A (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG367860.10448712644808444Clontech Universal Primer Mix Long (96% over 26bp)
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT366580.1041235546494286Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG361940.10280560687930107Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)