FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43630430
Sequences flagged as poor quality0
Sequence length150
%GC23

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3859130.8845042324817793No Hit
CTGTAGCCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1723230.39496058141072643No Hit
CTGTAGCCTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1692260.38786232452900415No Hit
CTGTAGCCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1231320.28221587547956783No Hit
CTGTAGCCTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1162360.2664103929298886No Hit
CTGTAGCCTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1088940.24958268804593492No Hit
CTGTAGCCTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950950.2179556790982807No Hit
CTGTAGCCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT913600.20939514004331383No Hit
CTGTAGCCTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT861560.1974676848245594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT842170.1930235388466261Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTAGCCTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT754230.17286788143046036No Hit
CTGTAGCCTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT739250.1694344978951617No Hit
CTGTAGCCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT737560.1690471535577348No Hit
CTGTAGCCTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT732160.16780948526063116No Hit
CTGTAGCCTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT696510.1596385825214191No Hit
CTGTAGCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT656840.1505463044943632No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT655920.15033544248819No Hit
CTGTAGCCATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620070.14211870018241854No Hit
CTGTAGCCTTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598440.13716115105902005No Hit
CTGTAGCCTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT526570.12068870281590166No Hit
CTGTAGCCTTATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523400.11996214568593526No Hit
CTGTAGCCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT508320.11650584236735692No Hit
CTGTAGCCATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503520.11540569276993144No Hit
CTGTAGCCTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485740.11133055530280127No Hit
CTGTAGCCTTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477560.10945571703052204No Hit
CTGTAGCCTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474640.10878645935875489No Hit
CTGTAGCCTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472820.1083693193030644No Hit
CTGTAGCCTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460570.1055616458513015No Hit
CTGTAGCCATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454520.1041749989628798No Hit
CTGTAGCCTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447620.10259353391658069No Hit
CTGTAGCCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436530.10005172995086228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)