FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34051463
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6647771.9522714780272437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1425690.418686856420824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA1335000.39205363951616407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1218120.35772912312167027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT865840.25427394999151726No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG752620.22102427728288798Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT705280.2071217909198204No Hit
AGAGTCAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534210.15688312716549066No Hit
AGAGTCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529350.1554558757137689No Hit
AGAGTCAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498030.1462580330248953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC487460.14315390795396954Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478640.14056371087491895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTAGGGAGTCATAAGTGG458180.13455515846705324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT443780.13032626527676652Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398600.11705811289224195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCACTTTGCTCGGGTCACG390140.1145736381429485Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374420.10995709641021885No Hit
AGAGTCAAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360450.1058544826693643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)