FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35630977
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT16595174.657511917228652Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1874550.5261012068234896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1464880.4111254092190624No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1139520.31981160662532493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT887890.24919047266090963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT774700.2174231708549558Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT737680.20703333506684368No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG706260.1982151654163174Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG701340.19683434445258124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG600510.16853593433601327Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA590930.1658472626220718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT567230.15919574700407457No Hit
AGAGTCAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560570.1573265869190171No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA524090.14708830465131506Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507400.14240417825197438No Hit
AGAGTCAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502260.14096161326140455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT492460.13821119752062933Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTAGGAATAATGGAATAG479780.13465249633766707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC458340.12863526026805272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444350.12470890147076236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGACGGACCAGAGCGAAAG438670.12311478295978244Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA419070.11761395147823199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408850.11474566077713783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG398660.11188578971606644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT384310.10785839523850273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCGCCGGGCAGCTTC357770.10040982036501553Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)