FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33035412
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT7428472.248638521596159Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1418550.4294028480710336Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1277080.38657910487085795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCACAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337270.10209347472342709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)