FastQCFastQC Report
Fri 30 Dec 2022
EGAF00002373694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344127911
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAATCCAACTACACATAACACATAATACCAATGTTTCAAAACATCG260695797.575549139343132No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAGGCATCAATGCCTAA231871626.737948669324878No Hit
CGAGTAATCCAACTACACATAACACATAATACTGACCACTCAAGTGCTCA207996666.044167106224639No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACGTTCAAACGTGAT149780314.3524603849991115No Hit
CGAGTAATCCAACTACACATAACACATAATACACAGTGGTCAACCACTGT85791622.493015453198738No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAGACATCAATGCCTAA53317241.5493436683198882No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACATTCAAACGTGAT50072841.4550647709595401No Hit
CGAGTAATCCAACTACACATAACACATAATACTAACCACTCAAGTGCTCA37934481.1023366250580007No Hit
CGAGTAATCCAACTACACATAACACATAATACCAATGTTTCCCAACTACA31610570.9185703626347239No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAGGCATCAATACCTAA26893240.7814896479001379No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACGTTCCCAACTACA25826750.7504985551724108No Hit
CGAGTAATCCAACTACACATAACACATAATACCAATGTTTCAAAACATCA24415150.7094789239574352No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACGTTCAAACGTAAT22374860.6501902137196887No Hit
CGAGTAATCCAACTACACATAACACATAATACTGACCACTCAAGTACTCA21765300.6324770326461546No Hit
CGAGTAATCCAACTACACATAACACATAATACTGACCACTCCCAACTACA20809270.6046957928966128No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAGGCATCCCAACTACA20404120.5929225543114984No Hit
CGAGTAATCCAACTACACATAACACATAATACCAATATTTCAAAACATCG20036440.5822381550446223No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAAGCATCAATGCCTAA19716960.572954397761709No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACGTTCAAACATGAT19517120.5671472547311049No Hit
CGAGTAATCCAACTACACATAACACATAATACTGACCACTCAAATGCTCA18845210.5476222473567394No Hit
CGAGTAATCCAACTACACATAACACATAATACACAGTAGTCAACCACTGT17591500.511190735702923No Hit
CGAGTAATCCAACTACACATAACACATAATACACAGTGGTCCCAACTACA15778460.4585056746530507No Hit
CGAGTAATCCAACTACACATAACACATAATACCAATGTTTCCAACTACAC15645410.4546393797159917No Hit
CGAGTAATCCAACTACACATAACACATAATACACAGTGATCAACCACTGT15497420.4503389438818289No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAGGCATCCAACTACAC14881960.43245431493058983No Hit
CGAGTAATCCAACTACACATAACACATAATACACAATGGTCAACCACTGT13889150.4036042865468125No Hit
CGAGTAATCCAACTACACATAACACATAATACACAGTGGTCAACCACTAT13418380.38992419885407087No Hit
CGAGTAATCCAACTACACATAACACATAATACTGACCACTCCAACTACAC10769820.3129597936041869No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAGACATCCCAACTACA8257700.23996019317363654No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACGTTCCAACTACAC8051880.2339792775483416No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACATTCAAACGTAAT6135590.17829387863863214No Hit
CGAGTAATCCAACTACACATAACACATAATACATCACATTCAAACATGAT5959030.17316322825090463No Hit
CGAGTAATCCAACTACACATAACACATAATACACAGTGGTCCAACTACAC5544300.1611116048067371No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5321920.1546494727653753Illumina Single End PCR Primer 1 (100% over 50bp)
CGAGTAATCCAACTACACATAACCAATCCGGAGAAGATCGGAAGAGCGTC5087510.14783776140726929No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAGACATCAATACCTAA4591060.1334114395620761No Hit
CGAGTAATCCAACTACACATAACACATAATACTTAAACATCAATGCCTAA3955100.11493110188321806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)