FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002388563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002388563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264416388
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGCGAT7764942029.36634169588611No Hit
ACCCGACG6974953426.378672868037214No Hit
TGATATTC4438410016.78568425191558No Hit
GTGATCGA3913772014.801548533368514No Hit
TATGCGAT44130801.6689888374089732No Hit
NATGCGAT38075971.4400003830322348No Hit
NCCCGACG26367410.9971927307319545No Hit
GATGCGAT24697640.934043467835284No Hit
AATGCGAT23880290.9031319949805834No Hit
GGATATTC22474380.8499616899690801No Hit
NGATATTC19885660.7520585297458945No Hit
TTGATCGA19070350.7212242079337382No Hit
NTGATCGA17576640.6647333825617495No Hit
TGATATTA11869430.44889161711111497No Hit
GTGATAGA9786880.37013137022354303No Hit
TGATATTT7260510.2745862332859641No Hit
ACCAGACG5667910.21435547330750168No Hit
AGATATTC5600880.21182045645370515RNA PCR Primer, Index 37 (100% over 8bp)
GTGATTGA5366170.2029439264558746No Hit
CATGAGAT5136570.19426065225578984No Hit
ACCCGAAG4334510.16392743402878646No Hit
ATGATCGA4326630.1636294192173898No Hit
GTGATGGA3161610.11956936647966011No Hit
TGATATTG3017920.11413513446829172No Hit
AACCGACG2880230.10892781728793603No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)