FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002388565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002388565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249888594
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTCGC8261114033.059187967578865No Hit
CCCAATAG5358122221.442043889366154No Hit
TATCGATA4526768018.11514454317191No Hit
GTGTCGCT3725994014.910620530363222No Hit
NGAGTCGC35861841.4351131208493655No Hit
TCCAATAG30084831.2039296999686189No Hit
TTGTCGCT25828861.0336150036523877No Hit
NCCAATAG24482820.9797493998465572No Hit
NATCGATA21365260.8549914046897235No Hit
GATCGATA19981980.799635536786445No Hit
NTGTCGCT17216010.6889474115013028No Hit
ACCAATAG13557420.5425385682069186No Hit
GCCAATAG12523240.5011529257713939No Hit
TATAGATA10118410.40491684066220324No Hit
AATCGATA8964590.35874346469771246No Hit
ATGTCGCT8242780.3298581927272759No Hit
AGAGTTGC7852030.314221224518955No Hit
AGAGTCGA7585490.30355487133598424No Hit
TATTGATA5990160.23971322196482486No Hit
AGAGTAGC4016900.1607476330032094No Hit
CATCGATA3912860.15658417766758895No Hit
AGAATCGC3885580.15549249118589223No Hit
CTGTCGCT2978320.11918591210289495No Hit
TGAGTCGC2830250.11326047158438932No Hit
CACAATAG2792740.11175940267205632No Hit
AAAGTCGC2742970.10976771512828633No Hit
GTGTAGCT2573100.1029698858524131No Hit
AGAGTCGT2534910.10144160481370348No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)