FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002388567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002388567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272134913
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGCTT7675536428.204894092365134No Hit
ATGAAGAC6064731722.285753904718575No Hit
CCACTACA5432539119.962668663538956No Hit
GATTCTGG4440056616.315644880155457No Hit
TCACTACA35999411.3228515813404653RNA PCR Primer, Index 43 (100% over 8bp)
NGCGGCTT35205031.293660912960477No Hit
GGCGGCTT31963201.1745350733443012No Hit
TATTCTGG30738021.1295140215985444No Hit
NTGAAGAC26181900.9620926514489524No Hit
ATGAAGAA25775840.9471713759858515No Hit
NCACTACA24010680.8823079602432343No Hit
NATTCTGG20570810.75590484782818No Hit
GCACTACA18215890.6693698283395192No Hit
GATTATGG11521620.42337897306105665No Hit
AGCGGCTT10776310.39599145442981065No Hit
ACACTACA10478420.3850450456535138No Hit
ATGAAGAT6526240.23981634432918203No Hit
AATTCTGG6421560.23596972285581014No Hit
TGCGGATT6385740.23465346396035558No Hit
GATTTTGG5989070.2200772379396979No Hit
TTGAAGAC4988530.18331091534734464No Hit
CGCGGCTT4846350.17808630089296923No Hit
ATGAAGAG4071920.14962872477887465No Hit
TGAGGCTT3306330.121495987543502No Hit
CATTCTGG2946840.10828599563040997No Hit
GTGAAGAC2830710.1040186269668383No Hit
CAACTACA2778680.10210670763879531No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)