FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002388607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002388607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270344959
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGAG6462797323.90574369836872No Hit
GCTGTCCA6160632222.788041703414933No Hit
CGCTATGT5449696720.15830707610864No Hit
TTGAGATC5406827919.99973633686286No Hit
TGCTATGT34807981.287539450661627No Hit
TCTGTCCA33573591.241879638673048No Hit
GTGAGATC27791441.0279991941702897No Hit
NAACCGAG26563180.9825661295204695No Hit
NGCTATGT26126500.9664134332906129No Hit
NCTGTCCA25607390.9472116696653479No Hit
NTGAGATC24223010.8960037608838862No Hit
GGCTATGT22901470.8471202897480327No Hit
TTGAGATA16594590.613830199067999No Hit
AGCTATGT12221960.4520875863640572No Hit
AAAACGAG9660060.35732347426533667No Hit
TTGAGATT9190550.3399564036257839No Hit
ACTGTCCA8195820.3031615618177663No Hit
AAACAGAG8009820.2962814631213449No Hit
ATGAGATC6426030.23769742272131658No Hit
GCTGTACA4560320.16868522412507791No Hit
CCTGTCCA4446670.16448133586245267No Hit
AAACCGAA3938530.14568535010116462No Hit
GATGTCCA3829670.1416586428748612No Hit
CTGAGATC3824290.14145963786955615No Hit
TTGAGATG3693290.13661397695971095No Hit
TAACCGAG3396610.12563984964113942No Hit
AAACTGAG3141770.11621337463148332No Hit
GCTGTCAA2962320.10957555898055418No Hit
CGATATGT2898580.10721783053480201No Hit
TTAAGATC2797950.10349554918092628No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)