FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002388732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002388732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268056430
Sequences flagged as poor quality0
Sequence length8
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACGCT9630474735.92704230224957No Hit
AAGGGTGA6310188823.54052391132718No Hit
CGTTAATC4469365616.673226603816218No Hit
TTACTCAG3170027011.82596888274607No Hit
NCCACGCT40913791.5263125753036404No Hit
NAGGGTGA33327311.2432945555530976No Hit
TCCACGCT32542101.2140018428209314No Hit
GGTTAATC27029781.0083615602878842No Hit
NGTTAATC22274670.8309694343090371No Hit
GTACTCAG21983530.8201082883928581No Hit
TGTTAATC21196360.7907424567282344No Hit
NTACTCAG14363770.5358487390136472No Hit
ACCACGCT13088080.48825838648974024No Hit
AGTTAATC10797890.4028215253034594No Hit
GCCAAGCT8469770.3159696635518126No Hit
GACACGCT6062730.2261736456014131No Hit
TTACTAAG4660270.17385406498176523No Hit
AAGAGTGA4601030.17164408255381153No Hit
GCAACGCT4328630.16148204316531412No Hit
TTAATCAG4224070.15758137195216693No Hit
GCCACGAT3951290.14740515644411142No Hit
CCCACGCT3891890.1451892051237122No Hit
CGTTAATA3882650.14484450158498344No Hit
ATACTCAG3442010.12840617179002198No Hit
GAGGGTGA3436470.12819949888909585No Hit
TTACTCAA2881040.10747886182025179No Hit
CGTTAATT2757080.10285446239808536No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)