FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002388735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002388735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258795671
Sequences flagged as poor quality0
Sequence length8
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTGAT8203181131.69752055087506No Hit
GAGCAAGA6779091726.19476467208758No Hit
ATATCCCG5319248220.55385308203243No Hit
CGCAGTTC204745107.911457684313429No Hit
TAGCAAGA53645612.0728944109733582No Hit
GCTGTGAT35623801.3765222525689003No Hit
NCTGTGAT33443261.2922650472001134No Hit
NAGCAAGA31484301.2165698088512462No Hit
NTATCCCG17935840.6930502326679182No Hit
TATGTGAT17722830.6848194149275395No Hit
TTTGTGAT17058950.6591667447173025No Hit
TGCAGTTC14452460.5584506087043473No Hit
ACTGTGAT11458420.4427593381189131No Hit
AAGCAAGA11276370.43572483096133396No Hit
NGCAGTTC7579280.2928673408914943No Hit
GGCAGTTC6944240.26832906335593226No Hit
GAGAAAGA6169120.23837802140052028No Hit
ATATTCCG5682220.21956395089777217RNA PCR Primer, Index 37 (100% over 8bp)
TTATCCCG5476790.21162602831946137No Hit
GAGTAAGA5134070.19838314837963422No Hit
AGCAGTTC3773610.14581426286686225No Hit
CCTGTGAT3429010.13249873874435866No Hit
ATATCACG3341610.12912155706035747No Hit
ATATACCG3315210.1281014472610711No Hit
CAGCAAGA2989050.11549845437715996No Hit
TCTGTAAT2948930.11394819660642623No Hit
GAGGAAGA2771890.1071072784675753No Hit
ATATCCAG2705110.1045268643616531No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)