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Rare frequency functional variants in these healthy individuals will be studied with respect to the genetic health of the participants and loss-of-function analysis of human genes. This data is part of a pre-publication release. 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CL:/software/sciops/pkg/biobambam2/2.0.79/bin/bam12auxmerge level=0 rankstrip=1 ranksplit=0 zztoname=0 clipreinsert=1 /tmp/aYvGjPr_Y_/amp_bamadapterclip_pre_auxmerge_out PP:bamsormadup' VN:2.0.79 @PG ID:bamsormadup'' PN:bamsormadup CL:/software/sciops/pkg/biobambam2/2.0.79/bin/bamsormadup threads=16 SO=coordinate level=0 verbose=0 fixmate=1 adddupmarksupport=1 tmpfile=out/bsfopt_25992_5#15.tmp PP:bam12auxmerge'' VN:2.0.79 @PG ID:bamstreamingmarkduplicates' PN:bamstreamingmarkduplicates CL:/software/sciops/pkg/biobambam2/2.0.79/bin/bamstreamingmarkduplicates level=0 verbose=0 tmpfile=out/bmdfopt_25992_5#15.tmp M=out/25992_5#15.markdups_metrics.txt PP:bamsormadup'' VN:2.0.79 @PG ID:scramble.4 PN:scramble PP:bamstreamingmarkduplicates' VN:1.14.9 CL:/software/sciops/pkg/staden_io_bin/1.14.9/bin/scramble -7 -I bam -O cram -r /lustre/scratch117/core/sciops_repository/references/Homo_sapiens/GRCh38_full_analysis_set_plus_decoy_hla/all/fasta/Homo_sapiens.GRCh38_full_analysis_set_plus_decoy_hla.fa @PG ID:samtools.1 PN:samtools PP:scramble.4 VN:1.8-59-g4da22a9 CL:samtools reheader -i /nfs/sf47/jillian/rt638397/25992/lane5/out/25992_5#15.cram.headernew /nfs/sf47/jillian/rt638397/25992/lane5/out/25992_5#15.cram @PG ID:scramble.5 PN:scramble PP:samtools.1 VN:1.14.9 CL:/software/pkg/staden_io_bin/1.14.9/bin/scramble -I cram -O bam s3_in/25992_5#15.cram @PG ID:MarkDuplicates VN:2.18.14-SNAPSHOT CL:MarkDuplicates INPUT=[/dev/stdin] OUTPUT=/dev/stdout METRICS_FILE=out/25992_5#15.markdups_metrics.txt TMP_DIR=[tmp/markduplicates_tmp] COMPRESSION_LEVEL=0 MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 SORTING_COLLECTION_SIZE_RATIO=0.25 TAG_DUPLICATE_SET_MEMBERS=false REMOVE_SEQUENCING_DUPLICATES=false TAGGING_POLICY=DontTag CLEAR_DT=true ADD_PG_TAG_TO_READS=true REMOVE_DUPLICATES=false 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ST:BC,FI,QT,RT,TC:1 @CO TY:checksum ST: PA:pass HA:crc32prod CO:0 BS:1 NS:1 SQ:1 ST:BC,FI,QT,RT,TC:1 @CO TY:checksum ST:25992_5#15 PA:all HA:crc32prod CO:33535484 BS:d5b1195 NS:7c92c155 SQ:16675c9e ST:BC,FI,QT,RT,TC:60bbfa0 @CO TY:checksum ST:25992_5#15 PA:pass HA:crc32prod CO:33522528 BS:444f55b4 NS:3c6a4583 SQ:74179c89 ST:BC,FI,QT,RT,TC:449e4b85