FastQCFastQC Report
Wed 16 Nov 2022
EGAF00002395047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002395047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208023249
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC117972775.671133902922552TruSeq Adapter, Index 4 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC47624552.289385933011747TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCC3832500.18423421509006427TruSeq Adapter, Index 4 (98% over 51bp)
AGATCGGAAGGGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2466550.11857088146911886TruSeq Adapter, Index 4 (98% over 50bp)
CACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTT2334550.1122254368789327TruSeq Adapter, Index 4 (100% over 51bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGC2131250.10245249077904749TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)