FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002396161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002396161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89193189
Sequences flagged as poor quality0
Sequence length150-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG15298061.7151601116089705TruSeq Adapter, Index 6 (97% over 35bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1017390.11406588455986254No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCCCGTATG932350.1045315242624636TruSeq Adapter, Index 6 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)