FastQCFastQC Report
Wed 1 May 2024
EGAF00002398854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002398854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36031156
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATCTCGTA705837819.58965180023644Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG28515187.914034176422205Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA8392902.3293451922552806Illumina Paired End Adapter 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC2076150.5762096558878099Illumina Paired End Adapter 2 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1391840.3862879114952626No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAGCTCGTA1127320.31287366966521973Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATATCGTA888040.24646447646586747Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAACTCGTA680360.18882547093409938Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAGATCGGAAGAGCGGTTCAGCAGGAAT568150.15768297858664318Illumina Paired End PCR Primer 2 (100% over 27bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGACCTCGTA525600.14587375436969052Illumina Paired End Sequencing Primer 2 (100% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG391400.10862821054089966Illumina Paired End PCR Primer 2 (100% over 31bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATCTCGTAT384410.1066882228258233Illumina Paired End Sequencing Primer 2 (100% over 35bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGGCACCCTCCAGCCTCTC378800.10513123697724269Illumina Paired End Adapter 2 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)