FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002398854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002398854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36031156
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA691439019.190030983185775Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAT23748776.591176258680127Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGA7486062.0776630092023693Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATC1706070.47349854664668545Illumina Paired End PCR Primer 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1161040.32223223701176834No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAA1098320.30482507971712036Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCG1093710.303545631452957Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA789270.2190520892529787Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA697180.193493653103997Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA565800.1570307652632627Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATCTCGTAT457530.12698177099841038Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA375240.10414320317671738Illumina Paired End PCR Primer 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)