FastQCFastQC Report
Wed 1 May 2024
EGAF00002398855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002398855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36031156
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG818383522.713217971690945Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT14041513.8970467669702296Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT2990190.8298901095485253Illumina Paired End Adapter 2 (96% over 30bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA1685190.46770356188405393Illumina Paired End Adapter 2 (96% over 29bp)
GCTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG672130.18654133661434566Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGT576230.15992548226873432Illumina Single End PCR Primer 1 (100% over 30bp)
CCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAA524940.14569057956397513Illumina Single End PCR Primer 1 (100% over 27bp)
CGGCAGTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT452950.12571064886177952Illumina Paired End Adapter 2 (96% over 30bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGCGT431570.11977689530693937Illumina Paired End Adapter 2 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAGAGTGTAGATCTCGGTGGTCGCCGT413730.11482562480093617Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGGTGTAGATCTCGGTGGTCGCCGT370380.1027943705164497Illumina Single End PCR Primer 1 (96% over 27bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT364610.10119297865436236Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)