FastQCFastQC Report
Wed 1 May 2024
EGAF00002398872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002398872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34236506
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTA10287853.00493572562574Illumina Paired End PCR Primer 2 (97% over 42bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6108701.784265018165113No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2283710.6670394461397434Illumina Paired End PCR Primer 2 (97% over 36bp)
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC1286930.3758940821823349No Hit
GTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGA1238420.36172499611963904No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA1156940.33792583857710246No Hit
GCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGG1134640.33141232344211763No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG1013750.29610206134936784No Hit
TGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGAC862990.25206719400630423No Hit
GCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGG813050.2374804251345041No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG788630.2303476879328749No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC744710.21751927606164018No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA738330.21565576814409743Illumina Paired End Adapter 2 (100% over 32bp)
GTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACC716270.20921235362043078No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC711820.20791257145223874No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA680110.19865052818181855No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC646770.18891238492619544No Hit
GTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCG638160.1863975254951542No Hit
GCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTA570880.16674598745561245No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG570280.16657073592731686No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT559260.1633519495242885No Hit
CTGGAGGACAGGGCACTGGCCACTGCAGTCACCATCTTCTGCCAGGAAGC557950.1629693170208432No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT536820.15679754236603466No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC517590.15118073088416206No Hit
GTGAATTCTTTGCCGAAATGGATTGCCAAAACGGTCACCAGCACATTTCC500770.14626784637427662No Hit
GGGCACTGGCCACTCCAGTCACCATCTTCTGCCAGGAAGCCTGCACCTCA473390.13827053496638939No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA468500.1368422350107806No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT421560.1231317237804582No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG397890.11621805098919848No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA393750.11500881544395913No Hit
GCCAAAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATC384530.11231578362581743No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT380250.11106565605730911No Hit
GTGATCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAG368380.10759859665586202No Hit
GGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCC361100.10547221144587592No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT353540.10326404218935191No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA352490.10295735201483469No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC345210.10083096680484861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)