FastQCFastQC Report
Wed 1 May 2024
EGAF00002398877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002398877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31916278
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG18051595.6559195279599965Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT2919590.9147651865922461Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT727870.22805604087042983Illumina Paired End Adapter 2 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)