FastQCFastQC Report
Sat 13 May 2023
EGAF00002399487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002399487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22298182
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATAGCCTTGTGTAGATCT6172422.768127015915468Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATAGCCTTGTGTAGATC580840.26048760387730263Illumina Single End PCR Primer 1 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA343920.1542367893490151No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308320.1382713622123992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)