FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122317
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2300327.367349311424817No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1205283.860210222088276No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT704432.256113008384479No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN550621.7634980689020368No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN448951.4378745015320353No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT418081.3390056166622415No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT407211.3041917268490033No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA397731.273829659192196No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA381981.2233863505851583No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA379191.2144506787747689No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA326671.0462422617562535No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC317511.016905074020351No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA258750.8287114985441901No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA250180.8012639331624559No Hit
CGCCTTGGCCGTACAGCAGACTCACGATAAGAGAA212440.6803921574907352No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA208090.6664601960659344No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200810.643144177865348No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA190610.6104761303865046No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178310.5710823084267229No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT167290.5357880061505605No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC154210.4938960393835731No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA153350.4911416745961412No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT150670.4825583052585628No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT135390.43362028903535416No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA122380.3919525147510647No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA120820.3869562251366533No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC115320.3693411014961005No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA114430.3664906542160838No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA113740.3642807568866326No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT112060.3589001373018819No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC110960.35537711257377136No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA107180.34327071850808233No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA100210.3209475527308726No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA93680.30003359684490716No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC88630.28385971059312687No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA85320.27325860891126685No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA80110.2565722826990341No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA77860.24936609575517155No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA75130.2406225889299517No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA75020.24027028645714063No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT73880.23661915173891698No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC72230.23133461464675112No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT70910.22710698497301843No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA70560.2259860225595287No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG70360.22534547260896315No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC66080.21163770366686022No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT65570.21000430129291806No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT64120.20536031415131778No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA63350.20289419684164037No Hit
CGCCTTGGCCGTACAGCAGACTCACGATAAGAGCC61550.19712924728655037No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA59920.19190876518944106No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA58960.18883412542672637No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT56740.18172402097544868No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA54260.17378120158843577No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC52650.16862477448638302No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC52120.16692731711738432No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA51410.16465336479287657No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA49800.15949693769082382No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC49210.15760731533665542No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA48860.1564863529231657No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC46540.14905597349660524No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA46510.1489598910040204No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG45750.14652580119187128No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC44750.1433230514390435No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC44000.14092098912442266No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA43590.13960786172576325No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC43100.13803851434687767No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC42560.13630902948035065No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC42110.13486779209157815No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG41500.1329141147423532No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA40630.130127722457393No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA40580.12996758496975164No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC40280.1290067600439033No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC38600.12362614045915261No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT38180.12228098556296495No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT38040.12183260059756905No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA37300.11946256578047648No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC36950.11834160336698675No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC36930.1182775483719302No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC35680.11427411118089548No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA35380.11331328625504714No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC34440.11030270148738902No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT34430.11027067398986073No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA34100.10921376657142756No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG33140.1061391268087129No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG31790.10181541464239538No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC31690.10149513966711259No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC31380.10050228724373597No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA31350.10040620475115114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)