FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340658
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC863072.5835329447073No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN700222.0960541306533025No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA587291.7580069555159492No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT490981.4697104582390657No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN403561.2080254847997012No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGAGAC327470.9802559854974678No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA281670.8431572462670527No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA248940.745182535895623No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT248500.7438654301038897No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA228870.6851045512590633No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211750.6338571622716244No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA205300.6145495887337166No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT203280.6085028757807593No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA200280.5995226090189418No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC196460.5880877360088941No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA184480.5522265374067025No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA176230.5275308038117041No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN169780.5082232302737963No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT163210.4885564460654158No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA161140.4823600619997617No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA157670.4719728867785928No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG156770.46927880675004746No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA156400.46817124051609No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG152540.45661663061588464No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC144490.43251958147167413No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT142610.42689194763426846No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG140010.4191090497740265No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA125050.3743274528550962No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA120300.36010869714888505No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA114230.34193862406747416No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGAGAA112340.33628105600752906No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT111760.33454487110024433No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA111420.333527107533905No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT110610.3311024355082143No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC110220.3299350008291779No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA110180.3298152639390204No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT100600.3011382787462829No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG98550.2950017631257076No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTGT97390.2915293933111381No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA94000.2813816918702842No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA93820.2808428758645752No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT90580.2711441877618122No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA84260.25222575911691647No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGAGAG84050.2515971404435892No Hit
CCACTACGCCTTGGCCGTACAGCAGCCCTAGATTC78930.23627081850342058No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG78240.23420535714820256No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA76640.22941588154189982No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA76620.22935601309682102No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC73940.221333641456264No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA73210.2191484432108884No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT71500.21402969115665235No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC71090.21280238803253732No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC70440.21085666356747682No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA67270.2013675150224896No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT65040.1946921833962052No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA61080.18283823127060597No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC61080.18283823127060597No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC58770.17592342586400644No Hit
TTCCCTTTGTCATCCTATGCCTCGCCTTGGCCGTA57670.1726306613846733No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC56730.16981684446597048No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT56280.16846980445169782No Hit
TAGCTTATCAGACTGATGTTGACATAAGTGGCCAC56240.16835006756154028No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA55970.1675418435529767No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT55820.1670928302148858No Hit
TCAGTGCACTACAGAACTTTGTCCGCCTTGGCCGT55250.16538657953014047No Hit
TAGCTTATCAGACTGATGTTGACTCGCCTTGGCCG51370.1537721011848564No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA50740.1518862451648747No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT50590.1514372318267838No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC49690.14874315179823855No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAAAC49470.1480845989023719No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA49210.14730630911634773No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC47950.14353459707638433No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT47740.14290597840305713No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC47410.14191814905925718No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT47290.14155893838878447No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAACA46310.13862538457992407No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA45430.13599117299645758No Hit
TAGCTTATCAGACTGATGAACTGCGGAACCGGCAT44230.13239906629173057No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT43900.1314112369479306No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTAC42100.12602307689084008No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA41860.12530465554989467No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41710.12485564221180379No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT41140.12314939152705844No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC40470.12114379861691918No Hit
TAGCTTATCAGACTGATGTTGACATAAGTGGCCCA38860.11632438878807709No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAATG38430.11503721721888321No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA37820.11321122964398032No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA37050.11090629450844715No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGCG36890.11042734694781686No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC36400.10896057004338668No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG35920.10752372736149585No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGGT35400.10596714778944748No Hit
TCGAGGAGCTCACAGTCTAGTCGCCTTGGCCGTAC35370.10587734512182931No Hit
TATTGCACTTGTCCCGGCCTGTCGCCAACCGGCAT33800.10117767218314475No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC33710.10090826418029024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)