FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975538
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2187605.502651465034418No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1299193.2679602106683423No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA736411.8523530651700475No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN672601.6918464871924253No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN548321.379234709868199No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT528271.3288012842538544No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT522961.3154446014602301No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA380830.957933240733707No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT350970.8828239096192767No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC339930.8550540832460916No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATAC338250.8508282401023458No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA332160.8355095587062681No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA316410.7958922792336534No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN289490.7281781736207779No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA260430.655081148765274No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA257110.6467300777907292No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT255090.6416490044869398No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN234910.5908885791055198No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC209570.5271487783540241No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA190440.479029504937445No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA190230.4785012745444768No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA189390.476388352972604No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA160010.40248640561352955No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA136460.34324914011638175No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT130440.3281065355179601No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA123940.31175654716418255No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC121280.30506562885325206No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGAGCA119020.2993808636717848No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC110060.2768430335718084No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA109570.27561049598821596No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC108640.2732711899622139No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA107420.2702024229173511No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA101230.25463220323890756No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA101010.2540788190177028No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA99840.25113582111402283No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT93270.2346097559625892No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC88070.2215297652795672No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA87470.22002053558537235No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA86060.21647384580401446No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT85700.21556830798749754No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC85240.2144112318886148No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA83040.20887738967656702No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC80530.20256377878918527No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC78790.19818701267602018No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC76370.19209978624276763No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA75340.18950894193439982No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT74500.18739602036252703No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC73760.18553463707302006No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA72140.18145971689869397No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC70480.17728418141142155No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT70470.17725902758318496No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC69310.17434118350774158No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC69250.1741902605383221No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC67110.16880734129569383No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG66240.16661895823911127No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC65550.1648833440907872No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA62960.15836850257751278No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT62910.15824273343632989No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC61150.15381565966669167No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA60530.15225612231602365No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG57160.1437792822002959No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA57100.14362835923087644No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA56940.14322589797909113No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA56070.1410375149225086No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC55710.14013197710599168No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT55350.13922643928947478No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA54070.13600674927519243No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT51440.12939129244897168No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA47890.12046168342498549No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATCA46390.11668860918949839No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC45130.11351922683168919No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC45090.11341861151874287No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA44550.1120603047939675No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44300.11143145908805299No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC43580.10962038345501918No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT43360.1090669992338144No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG42810.10768353868080245No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG42590.10713015445959768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)