FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821087
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1153164.087644230752189No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA474851.6832164339490414No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC460561.632562200314985No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN366061.2975849380043933No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA333741.1830191695612364No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAGAC325251.1529243869473007No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA230420.816777362768323No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN217690.7716529125120919No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT204350.7243661751658138No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA195450.6928180520487316No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA193570.6861539541318648No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC176800.6267087828202391No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149790.5309655462592965No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT134180.4756322651516951No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA133100.47180395358243116No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT113840.4035323972638915No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113720.40310702931175113No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC112180.3976481405926155No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAAGT111400.39488324890370274No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA109490.3881128089988008No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA101970.3614564173313336No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC101190.35869152564242085No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA96610.34245664880239424No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA91380.3239176955549403No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA91040.3227124863572091No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA88930.3152330998654065No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA84440.29931724898948525No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA82620.2928658350486887No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA79060.2802465858018558No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA75920.2691161243875145No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC75820.2687616510940641No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG73680.2611759226142264No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG70990.25164059102041164No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC69840.24756414814573247No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT67680.23990752500720464No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC67260.23841873717471315No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT66550.23590197679121558No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAATGCA64530.22874161626351827No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA61600.2183555487654227No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT58500.2073668766684615No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA54930.19471218009228358No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA53000.1878708455286916No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT52230.18514140116912384No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA51690.18322724538449187No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT50060.17744933070125096No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT48290.17117515340717956No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA47540.16851660370630187No Hit
GTCCAGTTTTCCCAGGAATCCCTCACCTTGGCCGT47490.1683393670595767No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT44480.1576697209267208No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA44030.15607459110619418No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT43990.15593280178881402No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG43810.15529474986060338No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT41220.14611389156023902No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC39880.14136394942800418No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG38930.13799645314022574No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA36560.12959543608545215No Hit
GTCCAGTTTTCCCAGGAATCCCTCGACTTGGCCGT36470.12927641012134686No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA36350.12885104216920643No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA36350.12885104216920643No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC35430.12558988786946307No Hit
CTGACCTATGAATTGACAGCCCGACTTGGCCGTAC35010.12410110003697157No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGACTG34620.12271865419251515No Hit
GTCCAGTTTTCCCATTCCGAAAATAAGGTTAATGC34140.12101718238395343No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC33940.1203082357970527No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG33240.11782692274290016No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA33240.11782692274290016No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC32820.11633813491040866No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG32480.11513292571267743No Hit
CTGACCTATGAATTGACAGCCCACCTTGGCCGTAC32240.11428218980839655No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGATCA32130.11389226918560116No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGCGGCATGTC32010.11346690123346072No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC31790.11268705998786993No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA31610.11204900805965928No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG31370.1111982721553784No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC30690.10878785375991594No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA30610.10850427512515566No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGCGGCAT30540.1082561438197404No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA30310.10744085524480457No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA29610.10495954219065205No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC29290.10382522765161088No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC28430.10077675732793778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)