FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853406
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1566585.490210646504563No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT788572.7636095248976136No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA637902.235573907113113No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN478391.6765577699072616No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN398421.396296215820672No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA376671.320071521543026No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT330471.1581597571463718No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA216410.7584269466034627No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA201050.7045965418170425No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN195790.6861624318446096No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAGAC188200.6595626419794449No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN185690.6507661370306224No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA184660.6471564158763247No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA178510.6256032264598869No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC175190.6139680087586554No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA168250.5896461982627078No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC154240.5405469813969691No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC153660.5385143228828986No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA146040.5118093955083853No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA144820.5075338034615473No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT131100.459450915852844No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA125950.4414023100813554No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA123540.4329562634970277No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG121280.4250359044594425No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA116140.4070223445244035No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT115490.40474436515518647No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA100300.3515097395884077No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC96920.3396642468684793No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA92080.32270206202692503No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT84350.2956116304514675No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA83680.2932635594093515No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT82460.2889879673625134No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC81710.2863595296288015No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA76280.2673296404367272No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT75550.26477129437591423No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA75090.26315918589923765No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC74070.25958451058138937No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA73810.2586733188337026No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA73390.2572013937028239No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA69040.2419564548472948No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA68070.23855700871169405No Hit
CTGACCTATGAATTGACAGCCCGACTTGGCCGTAC67400.23620893766957804No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT64940.22758766190300295No Hit
CCACTACGCCTTGGCCGTACAGCAGTAGTACATTC63520.22261115312717503No Hit
CTGACCTATGAATTGACAGCCCACCTTGGCCGTAC62140.21777482769714512No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT58860.2062797933417116No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA58860.2062797933417116No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG57260.20067245950979284No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA56690.19867484683217176No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT55640.19499503400497512No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA54120.18966806686465226No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA53740.18833632507957157No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT53410.18717981247673834No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC53040.18588311652810713No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT51530.18059119522423378No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC46020.16128093934056353No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA45820.16058002261157367No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA45590.15977396837323538No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44620.1563745222376346No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA42990.15066205089636736No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT42400.14859434654584733No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC42060.14740278810656457No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT41780.1464215046859788No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC41610.14582572546633743No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA41490.14540517542894352No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC41290.14470425869995365No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC41270.14463416702705467No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC40360.1414449959101509No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC39280.13766004557360573No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA38830.13608298293337856No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA38100.13352463687256563No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC37070.12991491571826794No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC36230.12697106545651057No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT35130.12311602344706643No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA35100.12301088593771795No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC34940.12245015255452606No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG34530.12101327326009689No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA34120.1195763939656677No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCTACG33980.11908575225537481No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA33660.11796428548899104No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA33630.11785914797964257No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA33550.11757878128804665No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA32270.11309291422251161No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA31290.10965842225046138No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA31250.10951823890466342No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT31250.10951823890466342No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC30890.10825658879248169No Hit
TGACCTATGAATTGACAGCCACGCCTGTTAATGCA30630.10734539704479489No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG30430.10664448031580505No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC30180.10576833440456773No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG29960.1049973260026789No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC29740.10422631760079008No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA29390.10299971332505785No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC29320.1027543924699114No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA29080.10191329239512359No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC28970.10152778819417917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)