FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713456
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1420663.8257084505646493No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT1096352.9523710527336258No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA887102.3888797928398775No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN832742.242493246183609No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT596521.606374223903555No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT502961.3544256347725678No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA391871.0552703465451052No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN385521.0381703728278993No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA344720.928299675558294No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA331350.8922954789285237No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA291000.7836365908199802No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA273010.7351911534699751No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN263800.7103894593069098No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA262900.7079658409847861No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT262110.7058384426798109No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA259860.6997793968745017No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA250050.673361957163354No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA228790.6161107065762998No Hit
TAGCTTATCAGACTGATGTTGACCGCGAACCGGCA183560.49431042134335235No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT181360.4883860210003835No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165500.44567648034607116No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC165360.44529947305151857No Hit
TAGCTTATCAGACTGATGTTGACCGCTCCAATTAC161370.43455476515677044No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC160950.4334237432731127No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT156110.42039006251858113No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC150400.40501355071932993No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT147730.39782348303036313No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA146390.3942149846396456No Hit
TAGCTTATCAGACTGATGTTCTGGCGCTTGGCCGT134550.3623309391574856No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAC122620.33020453184311327No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC121140.32621902615784326No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT108240.29148049687407096No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA107940.29067262410002975No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA102790.27680414147898885No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA101560.2734918631054199No Hit
TAATACTGCCTGGTAATGATGACGCCTTGGCCGTA92390.24879788531222669No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA91030.24513552873657316No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA87940.23681443916394865No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC80530.21685998164513057No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC78120.21037007036033278No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA76840.20692314652442362No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA72830.19612458044473935No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA72440.19507434583848576No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG71160.19162742200257657No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC70800.19065797467372714No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA70200.18904222912564467No Hit
TAGCTTATCAGACTGATGTTCTGGCGAGGTTAATG69630.18750727085496638No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC65840.177301144809579No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT64720.17428508645315846No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC63070.16984178619593177No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG59950.16143990934590313No Hit
TTCACAGTGGCTAAGTTCCGACGCCTTGGCCGTAC57800.1556501544652744No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAG57330.1543844871192765No Hit
TAACACTGTCTGGTAACGATGTCGCCTTGGCCGTA56720.152741812478726No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA55780.1502104777867302No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA55720.15004890323192197No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA54500.14676355395082102No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA54490.14673662485835298No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA54370.14641347574873648No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC54120.14574024843703548No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA52340.14094686997772427No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC50990.13731144249453878No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA50520.13604577514854085No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA50500.1359919169636048No Hit
TCAGTGCACTACAGAACTTTCGCCTTGGCCGTACA49980.1345916041552667No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA49240.1325988513126317No Hit
TAATACTGCCTGGTAATGATGACTCGCCTTGGCCG48610.13090231848714515No Hit
CTTCCTCTCTTTCTCTCCCTTGTGACGCCTTGGCC47470.12783240194578852No Hit
CTGACCTATGAATTGACAGCCAACGCCTTGGCCGT47410.12767082739098026No Hit
TTCACAGTGGCTAAGTTCCGTCGCCTTGGCCGTAC45950.12373917989064634No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT45950.12373917989064634No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT44010.11851493595184648No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGAGTG43810.11797635410248565No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA42340.1140177775096837No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC41470.11167494646496416No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA40840.10997841363947762No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT40600.10933211542024464No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA40380.10873967538594775No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG40290.10849731355373539No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA39830.10725857530020552No Hit
TAACACTGTCTGGTAACGATGTTCGCCTTGGCCGT39500.10636991524876019No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA38530.10375779327936026No Hit
GCCCCCATGTCTAACAACATGGCACACATAAGGTT37900.10206126045387369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)