FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118210
Sequences flagged as poor quality0
Sequence length35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1769425.674473496012135No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA857012.7484037316280814No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT733892.353561819120585No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA411641.3201163488026784No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA398971.2794840629720257No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT398831.2790350874379852No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN397171.2737115203915068No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA363271.164995301791733No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA322351.0337661671279357No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA304580.9767783439858124No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT284270.9116448218689569No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN283070.9077964601486108No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA227390.729232476324558No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA213040.6832124840854208No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT192470.6172451502624904No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT185950.5963357182486106No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGG177350.5687557925861312No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC167480.5371030174362855No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC164370.5271293466443889No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC160200.5137562896661867No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139550.4475323983952332No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA134570.4315616972557974No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA133580.428386798836512No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA120830.38749795555783606No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114110.36594712992389866No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA111730.35831454584521244No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA104560.33532058456614533No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA98950.3173294935235279No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA93130.29866493917985No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA92410.2963559221476424No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC90660.2907437279721379No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGC88680.28439393113356704No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT83550.267942184779088No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT82820.26560109806587756No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA82000.26297138422364114No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC75720.24283162455383056No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC72820.2335314170629945No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC72570.2327296750379224No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC72500.23250518727090222No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC67070.2150913504863367No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC66580.21351993611719544No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA64470.20675323342558713No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT63880.204861122246417No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC63620.20402731054034204No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA62050.1989923706228894No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA61950.19867167381286058No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA61780.19812648923581158No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT60150.19289913123234165No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA59610.19116736845818597No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA58150.18648519503176503No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC56450.1810333492612749No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT55780.17888468063408175No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT55540.17811500829001253No Hit
CATTATTACTTTTGGTACGCGACGCCTTGCGGCAT52440.16817340717911877No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT51910.16647371408596598No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC51160.16406848801074977No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA50070.16057289278143552No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG48490.15550588318297998No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC47520.15239512412570033No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC47120.151112336885585No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG46160.14803364750930822No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA45930.1472960448462419No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG45800.14687913899320446No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG45390.14556428207208622No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA44030.14120280545569414No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA43490.13947104268153845No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA42610.13664891075328475No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA41030.1315819011548292No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA40860.1310367165777802No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG40770.13074808944875427No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT40560.13007462614769372No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC40190.12888804795058703No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC39850.127797678796489No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA39310.12606591602233333No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA38020.12192892717296142No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC36300.11641294204046551No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT35900.11513015480035019No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT35750.11464910958530695No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC35450.11368701915522045No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT34630.11105730531298405No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA34180.10961416966785431No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA33430.10720894359263808No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA32660.10473957815541608No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT32560.10441888134538725No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC31530.1011157042020903No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG31310.10041017122002686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)