FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674941
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT848232.308145899485189No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC650771.770831150758611No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN557581.5172488483488578No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA474941.2923744898217413No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT461621.2561290099623368No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA355940.9685597673540882No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT336520.9157153815530644No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA302170.8222444931768973No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA269050.7321205973102697No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA258610.7037119779610067No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA247890.6745414416177021No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN244300.6647725773012411No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC232150.6317108220240815No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN202240.5503217602677158No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA188990.5142667596568217No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA178390.48542275916810634No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC162810.44302752071393803No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC154600.4206870259957915No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA150160.40860519937599No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT148010.4027547653145996No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC146440.3984825878837238No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA145960.3971764444653669No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA132600.3608221193210993No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA128740.3503185493318124No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA128260.34901240591345545No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN112370.3057736165015983No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT107370.29216795589371364No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA99110.2696914045694883No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA97510.26533759317496525No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC90320.24577265322082723No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC84780.2306975812672911No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA81630.2221260150843238No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA80780.21981305278098345No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC76270.20754074691267155No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAT73620.20032974679049267No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA73540.20011205622076653No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC72780.19804399580836807No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA72730.19790793920228925No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGC72360.19690112031730578No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA70940.19303711270466656No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA70370.19148606739536772No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC68250.18571726729762464No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG68210.18560842201276156No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGG68140.18541794276425116No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA68010.18506419558844617No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC65270.17760829357532543No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT61640.1677305839740012No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA58980.1604923725306066No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA57790.15725422530593008No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT57220.15570317999663125No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA55810.15186638370520777No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA54900.1493901534745728No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC54080.14715882513487974No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA53770.14631527417719087No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC51360.13975734576419052No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC50770.13815187781246013No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA50530.13749880610328166No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT50510.1374443834608501No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC49010.13336268527848474No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA48920.13311778338754282No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG48900.13306336074511127No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA48090.130859243726634No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT46520.12658706629575822No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG46380.12620610779873745No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT45500.12381151153174977No Hit
TACCCTGTAGATCCGAATTTGTGTCGCCTTGGCCG44230.12035567373734708No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC43250.11768896425820169No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA41540.11303582833030518No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC41460.11281813776057902No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA41410.11268208115450017No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAA40950.11143036037857479No Hit
TCCGGCTCGAAGGACCATCGCCTTGGCCGTACAGC40200.1093895112873921No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG39750.10816500183268249No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA39130.10647789991730479No Hit
ACCCTGTAGATCCGATAAACACTGCGGAACCGGCA39100.10639626595365748No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC39060.10628742066879442No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA38660.10519896782016365No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG38560.10492685460800595No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC38540.10487243196557441No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC37560.10220572248642905No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA37280.1014438054923875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)