FastQCFastQC Report
Wed 8 May 2024
EGAF00002406200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5881622
Total Bases205.8 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2023073.439646410462964No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1286702.187661838860097No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT994401.6906900851499806No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT834391.4186392801169474No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN634741.079192100410397No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN584410.9936204672792641No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA559480.951234200361737No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA518370.8813385151238893No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA435550.7405270178872426No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA425820.7239839622471488No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC386910.657828741799456No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN385530.655482450249268No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT380340.6466583537670391No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA367110.6241645586880625No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC352020.598508370650137No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT341120.579976067826188No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN323100.5493382607722835No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA307280.5224409185085339No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA281770.4790685290554204No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA253640.43124158607948626No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC243000.4131513381852829No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA239470.40714959240835263No Hit
GGTAGCGTGGCCGAGCCGCCGGTTAATGCACTACT237560.40390218888599094No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC218840.3720742339443099No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT216900.36877582408390064No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC208220.3540179902754716No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA190220.3234141874469321No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA186580.3172254184304942No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT185200.31487912688030617No Hit
GGTAGCGTGGCCGAGCCGCCGGTTATACGTGATGA163570.2781035571480112No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA159410.2710306782720821No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC152790.2597752796762526No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGTTTG147320.2504751240389131No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC143760.24442237192393526No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC136140.23146676205985356No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA135130.22974954867891884No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT134050.22791332050920649No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA115370.1961533740182555No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC114930.19540528106022453No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA114180.1941301226090354No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA111060.18882546345208856No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGAAAC105790.1798653500683995No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA103700.1763119085177524No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC103080.17525777753143607No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGAGC90780.15434517893193408No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA83730.1423586894907561No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA83030.1411685416029796No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA82210.1397743683630128No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGTGGT81630.13878824582742652No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT81210.1380741570947606No Hit
GGTAGCGTGGCCGAGCCGCCTTGGCCGTACAGCAG79440.1350647831499542No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC76980.13088226343005382No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA75900.12904603526034145No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC75390.12817892751353283No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA73300.12462548596288575No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT72860.12387739300485479No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACC72280.12289127046926851No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA72070.12253422610293556No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC72060.12251722399025303No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC71370.12134407821515901No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT71030.12076600638395328No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA69950.1189297782142409No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCAACCGGCAT67800.11527432398749869No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC63830.10852448525253747No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT63530.10801442187206181No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA63380.10775939018182398No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG63270.10757236694231626No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC61950.10532808806822334No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGTTTT60350.10260775003901985No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG59990.10199567398244905No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT59510.10117957257368801No Hit
TAGCTTATCAGACTGATGTTGACCGCTCCAATTAC59220.10068651130589487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)