FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4036310
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN533781.3224455009649905No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT468121.1597721681436757No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN367330.9100638949931992No Hit
CTGACCTATGAATTGACAGCCACGCCAACCGGCAT247870.61410050268686No Hit
TAGCTTATCAGACTGATGTTGACCGTCAACCGGCA246240.6100621607359197No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA236350.5855595828863491No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN219460.5437144322413293No Hit
TAGCACCATCTGAAATCGGTTACGTTCCAATTACG215500.5339034910599038No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198020.4905966092792675No Hit
TAGCACCATTTGAAATCAGTGTTCGTTCCAATTAC188260.4664161077816124No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA183490.45459838317671336No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA183260.4540285557848629No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA172070.42630521441613756No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT170760.42305967579298914No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC167560.41513164251506945No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA166190.4117374532679601No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG164230.40688153288523426No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCTACG153950.3814127259799173No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA152130.37690365705310047No Hit
GTCCAGTTTTCCCAGGAATCCCTCGGGAACCGGCA151110.3743765964457636No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAGCATG150780.3735590180139781No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC144910.3590160319697942No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCAACCGGCAT144890.3589664817618072No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC143380.35522544105878884No Hit
TAGCTTATCAGACTGATGTTGACCGTCAACCGTAC117750.29172684952345085No Hit
TAGCTTATCAGACTGATGTTGACCGTTGGTTAATG117380.2908101706756914No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA113010.27998345023053234No Hit
TCCCTGAGACCCTTTAACCTGTCGCCAACCGGCAT107340.2659359662662184No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG106350.263483230970862No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC106080.26281430316303755No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG104140.2580079329882987No Hit
CTGACCTATGAATTGACAGCCACTAACCAATTACG102450.25382094041339737No Hit
TCTCCCAACCCTTGTACCAGTGCGCCAACCGGCAT91200.225948948420711No Hit
TAGCTTATCAGACTGATGTTGACCGTCAACCGATG86830.21512222797555194No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGGT86270.21373482215191597No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT84640.20969648020097564No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC84140.2084577250013007No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA82360.20404775649045787No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT80380.19914228589974506No Hit
ATCACATTGCCAGGGATTTCCACGCCAACCGGCAT78780.19517826926078521No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC78220.19379086343714927No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT77150.1911399273098449No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC75910.188067814414651No Hit
GTCCAGTTTTCCCAGGAATCCCCGAAGGTTAATGC75320.18660608327903458No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC70320.17421853128228507No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC69740.17278157525066212No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC66530.16482876686874892No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC63790.1580403883745302No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTCGA61350.15199526300011645No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAGCAAC59840.1482542222970981No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG57010.14124286786693788No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA56890.1409455666190159No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA56510.14000411266726293No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT55820.13829463049171148No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT54280.13447926447671266No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTGCC53970.13371123625291417No Hit
TGGCTCAGTTCAGCAGGAACAGTCGTTCCAATCGT53900.13353781052495967No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA53540.13264590678119373No Hit
TGACCTATGAATTGACAGCCACGCCAACCGGCATG52520.13011884617385683No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC51770.1282607133743444No Hit
TAGCTTATCAGACTGATGTTGACCGTTGGTTACGT51000.12635303036684498No Hit
TAGCTTATCAGACTGATGTTGACCGTACCAATTAC50810.12588230339096848No Hit
TCTCCCAACCCTTGTACCAGTGCGAACCAATTACG50360.12476742371126102No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC46100.11421322941003045No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA45520.1127762733784075No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG45290.11220644598655702No Hit
TAGCTTATCAGACTGATGTTGACCGCTGGTTAATG44910.11126499203480407No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT44630.1105712891229861No Hit
TAGCACCATCTGAAATCGGTTCGGGAACCGGCATG44500.1102492127710706No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG43540.10787080278769472No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA42860.10618609571613677No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCTACG42630.10561626832428629No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGTACC41930.10388201104474136No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA41890.10378291062876736No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC41370.10249460522110541No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC41250.10219730397318344No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC40590.10056214710961249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)