FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795331
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1562105.588246973256476No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA975423.489461534251221No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT896613.2075271229060167No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN457361.63615686299762No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA437241.5641796982182075No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN359621.286502385585106No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA356191.2742319245913991No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA353411.2642867696169076No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT350071.2523382740720153No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA338881.2123072366027494No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT314311.124410669076399No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA295871.05844352600819No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA232480.8316725282265321No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA230820.8257340543928429No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC229980.8227290435372412No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN207400.7419514898235665No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN186550.6673628275148811No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA170750.6108400042785631No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC168610.6031843813845301No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA152830.5467331060257266No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT150090.5369310468062638No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA146160.5228718888746986No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT142150.5085265394330761No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA141440.5059865897813175No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT133440.47736743877558685No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC118910.4253879057614286No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA104390.3734441466860275No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGACCC94110.33666853764366367No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA93310.33380662254309057No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA84860.3035776442932876No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA82650.29567160382795454No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA82360.2946341596039968No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC81680.2922015317685097No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT80370.2875151457913213No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA79770.2853687094658915No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT76770.27463652783874254No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA76240.2727405090846129No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC73240.2620083274574639No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA70200.25113305007528625No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA69080.24712636893448395No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC63930.22870279047454486No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA63470.22705718929171537No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA62130.22226348149825548No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT61530.22011704517282568No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA61360.2195088882139539No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC61240.21907960094886794No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC60440.21621768584829487No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA59000.21106623866726337No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT57460.20555705209866024No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG54710.1957192189404403No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC54340.19439558320642528No Hit
CGCCTTGGCCGTACAGCAGGCACCCAACCAGACCA53050.18978074510675122No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT52680.1884571093727362No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA51690.18491548943577701No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA50080.17915588529587373No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT47170.16874566911753922No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC46960.16799441640363877No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT46260.16549024069063736No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC44610.15958754079570542No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG44270.15837122687796185No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA43550.1557955032874461No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG43000.1538279366558021No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA42450.15186037002415814No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC42220.15103756943274338No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG41520.14853339371974197No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA41400.148104106454656No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA40720.1456714786191689No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA38870.13905329994909368No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC37410.13383030489054784No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC36980.13229202552398983No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG36180.12943011042341676No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC35890.12839266619945902No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA34860.1247079505074712No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT34300.12270460993707005No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT34110.12202490510068396No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT33890.12123787844802639No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC33620.12027198210158296No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC33500.119842694836497No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT33300.11912721606135374No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT32430.11601488338948054No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG32390.11587178763445187No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG31390.11229439375873555No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC31260.11182933255489243No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC30470.10900319139307653No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA30390.10871699988301922No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC30300.10839503443420474No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA30100.1076795556590615No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA30030.10742913808776135No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC30030.10742913808776135No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA29270.10471031874221694No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA29140.10424525753837383No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA29080.10403061390583082No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC28940.10352977876323054No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC28640.10245656060051565No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA28230.10098982911147195No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)