FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4723114
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2113054.473849244375638No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1100352.3297129817319675No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN705161.4929980517091055No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN646241.3682498453350904No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA536041.134929201370113No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT500791.0602962367624411No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN423580.8968235786813531No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA411400.8710355075062767No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT359260.7606422373036094No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA303160.6418646680982081No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT297020.6288647701495242No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN275120.5824970559677366No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT274850.5819253992175502No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC269740.5711062659084662No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC251750.5330169883682673No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT248030.525140828699032No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT225630.477714490905788No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA221460.4688855699862421No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA209270.44307632633893657No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA207890.4401545251713171No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA189660.4015571082976189No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC185530.39281287726698955No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCA180040.38118919001319895No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA166000.3514630390035049No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA153220.3244046194946808No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA132370.28026001489695146No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA131900.2792649087021825No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCG128910.2729343395056736No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG127670.2703089529492619No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT121270.2567585707226207No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA119540.2530957330269818No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA112730.2386772794389464No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA112550.23829617493882216No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA111710.2365176872715755No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA105780.2239624112397033No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAT104520.22129467973883332No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC103590.21932563982152453No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA101040.21392665940309719No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA95800.20283228395503475No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA94700.2005033120098308No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC88410.18718582697771005No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT82960.17564682961283595No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC80300.1700149519998882No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC74940.15866650688507625No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA74630.1580101602459733No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAC74260.15722677877349564No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG73350.15530008380064508No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA72780.15409325288358486No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA71910.15225124779965082No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAA70020.14824965054834585No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC67880.14371874149131272No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA66950.14174970157400393No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC65740.1391878324342796No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA64170.1358637542943067No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA61010.1291732530699026No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT60070.12718304068036468No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA58970.12485406873516075No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT56730.12011143495583634No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC55130.11672383939917606No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT53450.11316686406468274No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA53130.11248934495335068No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCC52520.11119782414737396No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC51460.10895354209108651No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG51210.10842423028535833No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT50440.10679394992371559No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC49260.10429559820067862No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAACGCCTTG48970.10368159650603394No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT48020.1016702116442669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)