FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462027
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT482361.9591986602908906No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN477231.9383621706829373No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC476961.9372655133351502No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN304911.2384510811619855No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT231640.9408507705236376No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA206750.8397552098331984No Hit
CCACTACGCCTTGGCCGTACAGCAGTCATCCGCCT201880.8199747606342255No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA199130.8088051024623207No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA198420.8059212998070289No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA192970.7837850681572542No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT173830.7060442472807975No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA169650.6890663668595024No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA157470.6395949353926663No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA156340.6350052213074836No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN155290.6307404427327564No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA154580.6278566400774647No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT144740.5878895722914492No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140570.5709523088089611No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG121910.49516110099523686No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC118880.48285416853673824No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC118480.4812294909844612No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA117350.47663977689927856No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT103840.4217662925711213No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA100990.4101904650111473No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA96330.39126297152711975No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC85970.3491838229231442No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA85820.3485745688410403No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC84370.342685112714036No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA81770.33212470862423527No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAGAA76840.31210055779242063No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA73640.29910313737420424No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA72500.29447280635021467No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA71220.2892738381829281No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC70290.28549646287388397No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA69330.2815972367484191No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT69220.28115045042154285No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC64580.2623041908151292No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT63450.2577144767299465No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT61840.2511751495820314No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT56720.2303792769128852No Hit
CCACTACGCCTCCGCCTTGGCCGTACAGCAGTCAT56110.2279016436456627No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAGAT56070.22773917589043502No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT55390.224977224051564No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC54470.22124046568132683No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA51900.21080191240794677No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC50830.2064558999556057No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC49610.2015006334211607No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG49600.20146001648235376No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC49570.20133816566593302No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC47760.19398649974187934No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATG47170.1915901003522707No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA46550.18907185014624128No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC46490.18882814851339974No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA45790.1859849627969149No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC44030.17883638156689588No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC43960.17855206299524742No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA43020.17473407074739636No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA41780.1696975703353375No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG41110.16697623543527346No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA41080.16685438461885269No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA40220.16336132788145702No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA35640.14475876990788483No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA35280.1432965601108355No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG34730.14106262847645457No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA34400.139722269495826No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC33990.13805697500474204No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT33820.13736648704502427No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT33370.1355387247987126No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG33320.13533564010467797No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT33310.13529502316587105No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT33100.1344420674509256No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC32250.13098962765233688No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC32200.13078654295830225No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA31580.1282682927522728No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG31390.1274965709149412No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA31360.12737472009852044No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTT31350.12733410315971352No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC30900.12550634091340185No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA29220.11868269519383826No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG29170.11847961049980361No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC28820.1170580176415612No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT28730.11669246519229887No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA28300.11494593682360105No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG27710.11254953743399239No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA27260.11072177518768071No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCTCG26920.10934079926824523No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCCTA26810.10889401294136905No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCTACG26480.10755365396074047No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAAGG26380.10714748457267122No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA26350.10702563375625043No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA25900.10519787150993876No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT25350.10296393987555783No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA25060.10178604865015697No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA24810.10077062517998381No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA24740.10048630660833534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)