FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3383139
Sequences flagged as poor quality0
Sequence length35
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC912922.698440708466309No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA639391.8899312147682965No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT549641.62464504118808No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA456121.348215370400093No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN425311.2571461001158983No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN355821.0517451396469373No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAC342001.0108955026677886No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT292030.863192437555773No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA291930.8628968540754607No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG228880.6765314697386067No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA225600.6668363315843658No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA221870.6558110677687201No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA184620.5457062213524185No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT175430.5185420995117257No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAG170560.5041471840205206No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA160390.4740863440727679No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN148670.43944396018017584No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC148490.43891190991561385No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA139510.41236851338357666No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA132730.39232795341840815No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA132290.39102738610503446No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA131390.38836713478222445No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT131120.38756905938538144No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT130200.38484969136650904No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT128340.3793518386327018No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA127130.3757752785209239No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT126210.3730559105020515No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC120870.357271752653379No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA114630.3388273434818965No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT112670.33303390726777704No Hit
CCACTACGCCTTGGCCGTACAGCAGTCATCTTCTC106620.3151511067088878No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG104810.30980104571523664No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT103980.30734770282864526No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT101850.30105177469799493No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95300.28169105673754463No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC93660.27684348766042427No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT87340.258162611704692No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA87030.25724630291572415No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT75770.22396360303256826No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC74530.22029836787669674No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA72970.21568726558382614No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA70400.20809077013980212No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG68530.20256335905796363No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA68470.2023860089697763No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG67910.20073074148002787No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC67510.199548407558779No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA67050.1981887235493428No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC64780.19147897854625542No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA60100.17764567166764356No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC58300.17232516902202363No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA56220.16617703263152947No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA56190.16608835758743581No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATA55800.16493558201421815No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA54750.16183195547093987No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA54090.15988110450087922No Hit
CTGACCTATGAATTGACAGCTGTATCTTGCTTACG51740.15293489271354208No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGATGCA49630.14669808127895426No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC48970.1447472303088936No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC48630.14374224647583206No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT48290.1427372626427705No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA47970.1417913955057714No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA47020.13898335244280532No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG46800.13833306878611845No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT46800.13833306878611845No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA45750.13522944224284014No Hit
CTGACCTATGAATTGACAGCTATTCCAATTCATGT44770.13233272413578043No Hit
CTGACCTATGAATTGACAGTCCGCCTTGGCTACGT43390.1282536721074718No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC43220.12775118019094103No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC42620.12597767930906772No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC42400.12532739565238082No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT42260.12491357877994372No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC41490.12263758598153962No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41100.12148481040832199No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT40190.1187950007374808No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC40110.11855853395323102No Hit
CTGACCTATGAATTGACAGTCACGCCTTGCTTACG38730.11447948192492238No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC38420.1135631731359545No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC38180.11285377278320517No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA37480.11078468842101966No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA37180.109897937980083No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC37100.10966147119583322No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG36440.10771062022577257No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA36430.10768106187774135No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG36010.10643961126043003No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA35890.10608491108405538No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA35770.1057302109076807No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA35430.10472522707461916No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCG35300.10434096855021328No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT34930.10324730967305806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)