FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851714
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT2550788.944725873632489No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1163964.081615477568929No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1151234.036975657446715No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA627172.199273840223809No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC356611.2505110961337638No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT288281.0109008126340862No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA270280.9477808784471374No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA262990.9222173051014233No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA238590.8366547276480041No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA200100.7016832683782455No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA188460.660865710937352No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA164060.5753031334839329No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG155420.5450055650741975No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT151980.5329426443184695No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA151520.531329579333692No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA139600.48953015624989044No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC137500.4821661639280797No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC128330.45001006412283984No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA123940.4346158135072451No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT121610.42644528869304565No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC110660.3880473287293186No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAA97290.3411632442804573No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT95250.3340096517392698No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA94370.3309237882901301No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC90070.31584513734547015No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT85580.30010022042883683No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC81340.28523196926480004No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA79850.28000704137932486No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78870.27657051162914653No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA77310.27110011733294437No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT71220.24974453959969337No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG70700.24792107483429265No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT69700.2444144118239066No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA68160.23901415078791213No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC66820.23431522235399482No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT63690.22333936713148655No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA63510.22270816778961702No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG59640.2091373819394231No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA58760.20605151849028336No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC54880.19244566600998558No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA54240.19020140168333852No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT52970.18574793966014824No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA50970.17873461363937618No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG49960.17519288399888627No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGATG49540.17372008553452414No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT48820.1711952881670462No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA48510.17010822263382655No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA48030.16842502438884122No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACCTCACC46920.16453262844731276No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAA45490.15951810034246072No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG45390.1591674340414221No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA45080.15808036850820242No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA43960.15415290593657008No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT42570.1492786443521335No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC42420.14875264490057558No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA41510.1455615815611243No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC41300.14482518232894323No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT39470.13840798901993678No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC39450.13833785575972907No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC39410.13819758923931363No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39300.13781185630817114No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38870.13630399121370515No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA38820.13612865806318586No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC38660.1355675919815241No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA37540.13164012940989173No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC37290.13076346365729524No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT37010.12978159801438713No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC37010.12978159801438713No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC36510.1280282665091941No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC35270.12368000437631543No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC34140.11971747517457922No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC33980.11915640909291746No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG33970.11912134246281358No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACC33350.11694721139637425No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA33160.1162809454244009No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAC31760.11137161720986045No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA31520.1105300180873678No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC30000.10519989031158104No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA29630.1039024249977382No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC28850.10116722784963708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)