FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264217
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1309934.012999135780495No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA585911.7949480687098927No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT386081.1827645037079335No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA365301.119104520318349No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT301110.9224570547852671No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC256040.7843841264229675No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT248510.7613158071292442No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC238780.7315077398346985No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG221140.6774672149553782No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC219980.6739135296458538No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAC197160.6040039617464158No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA176580.5409566827205422No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT172320.5279060797734955No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA169570.5194813947724676No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA169550.5194201243360965No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC140780.43128260161625287No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC138360.42386887881534835No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA134580.41228876634120837No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC131290.40220977955816056No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA131020.4013826286671505No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC126210.3866470887198982No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC125710.3851153278106204No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC118460.3629047946260926No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT118030.3615874802441137No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC107870.3304620985675891No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAG98500.3017568991277234No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC91540.28043478727057664No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG89580.27443028450620777No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG86540.26511717817779884No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT85180.26095078850456327No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83980.25727456232229656No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC83210.25491565052200876No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA81850.25074926084877325No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG77820.23840326791999428No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA76360.23393052606490317No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG72510.22213596706346425No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA71250.21827592957208422No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA68550.21000442066198416No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA66620.20409182355217192No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG66000.2021924400246675No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA65720.20133465391547192No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA63160.19349203805996967No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT63060.1931856858781141No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA62860.192572981514403No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG62080.19018343449592967No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG61400.18810023965931186No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT57420.1759074228214607No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT56850.17416121538488405No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC56560.17327279405750293No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC56350.17262945447560624No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA55740.17076070616628736No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA54910.16821798305688623No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGAT54840.16800353652958736No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCTCCA53170.16288745509259953No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA52850.16190712811066174No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC52520.16089616591053843No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT51980.15924186412851843No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA49770.15247148090951063No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA48690.1491628773454706No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA48320.14802937427260504No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC47340.14502712289042058No Hit
GCCCGGCTAGCTCAGTCGGATCTCGTACTCTGAGC46370.14205550672642167No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG46360.14202487150823612No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC46310.14187169541730835No Hit
GCCCGGCTAGCTCAGTCGGATCTCGTACCCTGAGC46280.14177978976275168No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45950.14076882756262835No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTCGC44610.13666370832576388No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC44440.13614290961660944No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA40070.1227553192695216No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT39690.12159118097847049No Hit
GCATTGGTGGTTCAGTGACGAGGCCTCTATTCTAT39460.12088657096020271No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT39280.1203351370328627No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC38870.11907909308725491No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG38320.11739415608704937No Hit
TAGCTTATCAGACTGATGTTCTGTATTGGTTACCA38090.11668954606878158No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATTTG37780.11573985430502935No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA37400.11457571601397823No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA37190.11393237643208157No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACATA36730.112523156395546No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA36620.11218616899550489No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC36270.11111393635901046No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG36190.11086885461352601No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT35430.10854057803142377No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATGGT35020.10728453408581598No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA34980.10716199321307376No Hit
TAGCACCATCTGAAGCTAACCTATTCCAATTACGT34740.10642674797662043No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG34400.10538515055831153No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA33590.10290369788528153No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC33580.10287306266709598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)