FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3417067
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2258136.608386666108683No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1120453.2789816529790023No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT792552.3193867723401382No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA455541.3331316008729124No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA401351.174545304496517No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA381281.1158107230557668No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN380531.1136158582784594No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA361661.0583930604814011No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN358741.0498477202817502No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA334550.9790560149976573No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTC332940.9743443719423703No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA309660.9062157692547439No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT298690.8741122137786587No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC282350.8262934264970514No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT260920.7635788235934502No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT206650.6047584083074754No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA199190.5829268199891896No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT196660.5755228094737388No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC179500.5253043033689418No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA173830.5087111256524968No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG158360.4634383815125662No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA158240.463087203148197No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA155980.4564733439525769No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154420.4519080252157771No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC150060.4391485446436959No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA148950.4359001447732807No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN141970.4154732699124717No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA119420.34948100227475787No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC113400.3318635543289025No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA110330.3228792411737903No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT98960.2896050911498077No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA94770.27734311326058286No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA91070.2665151136925322No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA90750.26557863805421433No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA89820.262857005730353No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC89160.2609255247263223No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT87710.25668211949019437No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC86690.25369710339305607No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGAG86420.2529069520732254No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA83980.24576632533105147No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA80680.23610892031089822No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT79950.23397258526098552No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA79840.23365067176031373No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT79340.23218742857544203No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC77190.22589548288049371No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT70060.2050296350642232No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA69050.20207388383078234No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA67810.19844504073230051No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA64840.1897533762141626No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA63260.18512952774996802No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTG62430.182700544063081No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT62140.1818518630158554No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA61780.1807983279227478No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC61210.17913023069199405No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA60020.17564771191199938No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC59680.17465270654628665No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT59560.1743015281819174No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC54860.1605470422441234No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC53280.15592319377992883No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC53170.15560128027925704No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT52870.15472333436833402No Hit
GTCCAGTTTTCCCAGAGGCTTTTATTCCAATTACG52260.15293817768279053No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC51030.14933859944800612No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC50540.14790462112683186No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA50180.14685108603372424No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA48820.1428710645708732No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT48600.14222723756952965No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC48580.14216870784213478No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT48450.14178826461406813No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA48060.1406469349298682No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT47910.14020796197440671No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC47780.13982751874634006No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG47340.13853986474365296No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA46310.13552558378281726No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA45820.13409160546164298No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA45370.13277468659525846No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC45350.1327161568678636No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG42370.12399522748602822No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC42350.12393669775863335No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC41610.12177109784502324No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA40380.11817151961023883No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGCT39920.11682533588015687No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC39190.11468900083024419No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC38960.11401590896520321No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA38780.11348914141864938No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA38370.11228928200705458No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC37920.11097236314067005No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC37290.10912867672773172No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT36830.10778249299764973No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT36670.10731425517849079No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC36650.10725572545109592No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA36460.10669969304084469No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT36020.10541203903815757No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA35970.1052657147196704No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA35970.1052657147196704No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA35780.10470968230941916No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA34370.10058333652808095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)