FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3168260
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN424761.3406727983183198No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA376931.1897066528630857No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC360701.1384797964813493No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA352391.1122508885003124No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT317871.003295184107365No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA285820.9021355570565546No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA271970.8584207104214931No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA271880.8581366428260307No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT247870.78235372096987No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC229600.7246879990909837No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA217240.6856760493141345No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211790.6684741782555724No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT205130.647453176191348No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTGAA172710.5451257156925252No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172090.5431688055904503No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC170300.5375190167473629No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT165740.5231262585772632No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTCTT150070.4736669339006268No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149690.47246753738645186No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT126450.3991149716248035No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA114020.3598820803848169No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC110750.3495609577496796No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA106370.33573633477050496No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA98820.31190621981781796No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC96620.3049623452620681No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA89270.28176349163263115No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA83070.2621943906118816No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT70690.2231193147027075No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGAGAA68280.21551261575754516No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC65610.20708527709215785No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA64070.20222456490313293No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG62790.19818449243433306No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC62460.19714291125097058No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT62310.19666946525853304No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT62150.1961644561999331No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA61650.19458630289180812No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA58590.18492800464608333No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTGTT54050.17059837260830865No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGACTT53660.1693674130279712No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG53490.16883084090320868No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA52360.16526421442684627No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT52340.1652010882945213No Hit
AAACCGTTACCATATGACTCAAGCGGAACCGGCAT49420.1559846729750715No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC49250.155448100850309No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC48090.1517867851754591No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC47580.1501770688011716No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC46190.1457898026045842No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC45820.14462196915657174No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA44450.14029782909230934No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44240.13963500470289686No Hit
AAACCGTTACCAAATGACTCAAGCGGAACCGGCAT43760.1381199775270969No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT43010.13575274756490943No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC42920.13546867996944698No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC42180.133133013073422No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA41840.13205986882389703No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG40690.1284301162152096No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA40680.1283985531490471No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC40580.12808292248742212No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTCAA40070.12647320611313465No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG38620.12189656151957225No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT36820.11621520961032238No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC36670.1157417636178849No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC36380.11482643469917242No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT35730.11277483539860995No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT34100.10763005561412259No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT33770.1065884744307601No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC33440.10554689324739762No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC33280.10504188418879765No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA33110.10450531206403514No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)