FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5344593
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1873473.505355786680108No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1238472.3172391237274756No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1126402.107550565590308No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN832351.5573683533994076No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN663051.2405996116074693No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT598441.1197110799643677No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA583041.0908969120754377No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA503820.942672341935111No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN473070.8851375586503967No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA466810.8734247865085331No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT439390.8221205992673344No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA386930.7239653234586806No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN371850.6957498915258842No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA346150.6476639100489037No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA327050.6119268576671787No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA313270.5861437905561752No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA305250.5711379706555766No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA252560.4725523533784518No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA247430.46295386758168483No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC239030.44723704873317766No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT218480.4087869740502224No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC214170.4007227491410478No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT208660.3904132643963722No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA206980.38726990062667077No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC175880.32908024988993545No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA172900.32350452129844126No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC157940.29551361534919496No Hit
TCAACTTAACTTGACCGCTCTGACCACGCCTTGGC144260.26991765322448313No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC144100.2696182852464163No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC142500.2666246054657483No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA142040.2657639225288062No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC141670.2650716340795267No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT140580.26303218972894665No Hit
CCGGGCGGAAACACCACGCCTTGGCCGTACAGCAG139930.2618160073180502No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT139000.26007593094553694No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA135670.25384533490202155No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA129870.24299324569709985No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC125960.23567744073309232No Hit
GACCCCTTATTTACCCCACGCCTTGGCCGTACAGC121130.22664026989520064No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT120110.22473179903502474No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA114480.21419778830679906No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAT114050.21339323686574452No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC108640.20327085710736068No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC108500.2030089101265522No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC106300.19889260042813364No Hit
GCCCACCCAGGGACGCCACGCCTTGGCCGTACAGC101140.18923798313547915No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA100400.18785340623692018No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG97640.1826893086152678No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC91900.17194948240212118No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA91870.17189335090623364No Hit
ACCTCTTTACAGTGACCACGCCTTGGCCGTACAGC91820.17179979841308776No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC91530.1712571939528417No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA89890.16818867217765693No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT87680.16405365198060917No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA87480.16367944200802567No Hit
ACCCCGTCCGTGCCTCCACGCCTTGGCCGTACAGC86630.1620890496245458No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA85990.16089157771227858No Hit
GGCTCACATCACCCCATAAACACCACGCCTTGGCC85250.15950700081371957No Hit
TCCCACTTCTGACACCACGCCTTGGCCGTACAGCA82680.15469840266602153No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAG80050.1497775415265484No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA79900.14949688404711078No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT78850.14753228169104737No Hit
AACCGGGCGGAAACACCACGCCTTGGCCGTACAGC75980.14216236858447406No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA74630.13963645126953542No Hit
CCCGGCATCTCCACCACGCCTTGGCCGTACAGCAG74450.13929966229421026No Hit
CAACTTAACTTGACCGCTCTGACCACGCCTTGGCC73940.13834542686412232No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA73350.13724150744500097No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG73210.13697956046419252No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA73140.1368485869737883No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC69060.12921470353308473No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA68890.12889662505638877No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG68430.1280359421194467No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA67690.12665136522088774No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT67470.12623973425104587No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA67430.12616489225652916No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC66760.12491128884837442No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA66180.12382607992788225No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAAGC65130.12186147757181885No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT64760.12116918912253936No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA64030.11980332272260956No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA62870.11763290488162523No Hit
ATCCTATATATCTTACCACGCCTTGGCCGTACAGC62470.11688448493645821No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA62370.11669737995016645No Hit
CGACCCCTTATTTACCCCACGCCTTGGCCGTACAG62260.11649156446524551No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61780.11559346053104512No Hit
ACCCCTTATTTACCCCACGCCTTGGCCGTACAGCA61220.1145456726078113No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG60760.11368498967086922No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC60270.11276817523803964No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA60150.11254364925448954No Hit
ATCCCGGACGAGCCCCCACGCCTTGGCCGTACAGC58140.1087828390300253No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC57990.10850218155058766No Hit
TCCCACCGCTGCCACCACGCCTTGGCCGTACAGCA57220.10706147315614116No Hit
GGAGCGCTGAGAAGACGGTCCGCCTTGGCCGTACA54320.10163542855368034No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT53620.10032569364963806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)