FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673292
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC956582.6041490847991393No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN665121.8106918807434857No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA633261.723957692445904No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA590091.6064336840087856No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT516371.4057417705970556No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN461421.2561484357900217No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT410271.1169000449732827No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT358540.9760726890211833No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA354230.9643393446532429No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT344870.9388581141929364No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGT340030.925681922373718No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN274390.7469866267097742No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN231250.6295442888831054No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA223810.6092899774915798No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC203020.5526922444499376No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA199340.542673982901441No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT197150.5367120283386129No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA194960.5307500737757848No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC187540.5105502094578922No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA183410.49930688875265017No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA169280.4608400312308415No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA167720.45659315948745705No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATCA164530.4479088512429723No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG154840.4215292440677191No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGC119550.32545738264205515No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC115450.3142957325472628No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT114350.3113011434974404No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGAC112460.30615589503910934No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC109460.29798883399413933No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGTG103780.2825258650823294No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA103680.2822536297141638No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA90920.2475163967362246No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG87660.2386415237340239No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG86460.23537469931603586No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA84790.2308283686676692No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA83100.22622759094566947No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC81680.22236184871771697No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA80860.2201295186987585No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA79240.2157193057344747No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT78270.2130786226632677No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA78220.2129425049791849No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA77930.21215302241150447No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT77120.20994791592936257No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA77000.20962123348756373No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC75650.20594605601732724No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT72700.19791511265644007No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT72190.19652671227879515No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA66750.18171710825058285No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA65650.17872251920076052No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC64020.17428508269966014No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT63960.17412174147876075No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA61280.16682583361192085No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT60080.16355900919393285No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA58520.15931213745054845No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA57440.15637199547435923No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA55570.15128119408966126No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA54020.14706154588309342No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA52730.14354970963375632No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA52590.1431685801183244No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC52370.14256966230835993No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT48560.13219749478124798No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA46790.12737892876471568No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC43310.11790513795255048No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC43170.11752400843711854No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC42460.11559113732314229No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG41020.11167094802155668No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT40190.10941139446578164No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA40110.10919360617124912No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA39980.10883970019263374No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATCG38980.10611734651097707No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGG38190.1039666871024683No Hit
GGCCGCCGGTGAAATACCACTACCGCCTTGGCCGT37770.10282329855617249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)